EXPRESSION INFORMATIONTo the right, in Figure 1, is data from BioGPS showing where the MCM6 protein in expressed in your body; as you can see, it is highly expressed in cancerous cells (turquoise lines) and immune cells (purple lines) as well as thymus, bone marrow, tonsil and fetal liver cells [1]. In addition, it is expressed in all of the cell types studied. This is due to the gene ontology; MCM6 is involved in DNA replication which is common to all cells, though at varying levels. Click on the image to enlarge it.
Below is information on MCM6 expression in model organisms, though I was not able to find information for all organisms. |
Figure 1: Expression data for Homo sapiens
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EXPRESSION DATA IN VARIOUS MODEL ORGANISMS:
Danio rerio Expression of MCM6 in Danio rerio, also known as the zebrafish, has been looked at in one published paper and two direct submissions to ZFIN, the zebrafish database. From this information, I gathered that MCM6 is expressed primarily in the newly developing fish in regions where there is cell proliferation, especially in the central nervous system [2]. Mus musculus The MCM6 gene is on chromosome 1 in Mus musculus, the common house mouse. There have been 52 studies on the expression of MCM6, as found by Mouse Genome Informatics. I chose one that I found particularly interesting to describe. Below is an image of MCM6 expression overlaid by Proliferating Cell Nuclear Antigen (PNCA) expression [3]. These are expressed in the same tissues (yellow), for the most part, leading to the conclusion that these are cells are dividing. Drosophila melanogaster Multiple assays were done to determine that in Drosophila melanogaster, or fruit flies, MCM6 is expressed in unfertilized eggs, embryos and adults and especially in the central nervous system and ovaries [4]. Click the image below for a detailed image of the levels of MCM6 expression by tissue [5].
The image below is an example of microarray data done in Saccharomyces cerevisiae, also known as yeast. There are 361 expression studies done in yeast on the Saccharomyces Genome Database. The first 100 studies were able to show that MCM6 is over expressed during the mitotic cell cycle, and underexpressed during sporulation [8].
Arabidopsis thalianaAccording to The Arabidopsis Information Resource, MCM6 is primarily expressed in the shoot apex, which contains the apical meristem, produces cells which allow the shoot to grow longer and periodically cells for leaves. It has been observed by these Arabidopsis researchers that MCM6 is expressed primarily within the nucleus of a cell, which makes sense because it is involved in replication [7].
Dictyostelium discoideum
I could not find much expression data from dictyBase [6]. I proceeded to do a literature search and could not find any papers with the topical keywords "Dictyostelium discoideum" and "MCM6".
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Image 1: Danio rerio
Image 2: Mus musculus
Image 3: Drosophila melanogaster
Image 4: Saccharomyces cerevisiae
Image 5: Arabidopsis thaliana
Image 6: Dictyostelium discoideum
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REFERENCES
[1] Swallow, Dallas M. "Genetics Of Lactase Persistence And Lactose Intolerance." Annual Review of Genetics 37.1 (2003): 197-219. Web. 7 Mar. 2013.
[2] ZFIN. "ZFIN Search Expressions." ZFIN Search Expressions. University of Oregon, n.d. Web. 24 Apr. 2013.
[3] Barton, Kirston M., and Edward M. Levine. "Expression Patterns and Cell Cycle Profiles of PCNA, MCM6, Cyclin D1, Cyclin A2, Cyclin B1, and Phosphorylated Histone H3 in the Developing Mouse Retina." Developmental Dynamics 237.3 (2008): 672-82. Web.
[4] "Gene DmelMcm6." FlyBase Gene Report: DmelMcm6. N.p., 7 May 2013. Web. 11 May 2013.
[5] Chintapalli, Venkateswara R., Jing Wang, and Julian A T. Dow. "Using FlyAtlas to Identify Better Drosophila Melanogaster Models of Human Disease." Nature Genetics 39.6 (2007): 715-20. Web.
[6] DictyBase. "Gene Summary for Mcm6." Gene Summary for Mcm6. National Institutes of Health, n.d. Web. 16 May 2013.
[7] Carnegie Institution for Science Department of Plant Biology. "Locus: AT5G44635." tair. National Science Foundation, 11 Feb. 2013. Web. 16 May 2013.
[8] "SPELL - S. Cerevisiae." SPELL - S. Cerevisiae. Stanford University, 2007. Web. 16 May 2013.
[1] Swallow, Dallas M. "Genetics Of Lactase Persistence And Lactose Intolerance." Annual Review of Genetics 37.1 (2003): 197-219. Web. 7 Mar. 2013.
[2] ZFIN. "ZFIN Search Expressions." ZFIN Search Expressions. University of Oregon, n.d. Web. 24 Apr. 2013.
[3] Barton, Kirston M., and Edward M. Levine. "Expression Patterns and Cell Cycle Profiles of PCNA, MCM6, Cyclin D1, Cyclin A2, Cyclin B1, and Phosphorylated Histone H3 in the Developing Mouse Retina." Developmental Dynamics 237.3 (2008): 672-82. Web.
[4] "Gene DmelMcm6." FlyBase Gene Report: DmelMcm6. N.p., 7 May 2013. Web. 11 May 2013.
[5] Chintapalli, Venkateswara R., Jing Wang, and Julian A T. Dow. "Using FlyAtlas to Identify Better Drosophila Melanogaster Models of Human Disease." Nature Genetics 39.6 (2007): 715-20. Web.
[6] DictyBase. "Gene Summary for Mcm6." Gene Summary for Mcm6. National Institutes of Health, n.d. Web. 16 May 2013.
[7] Carnegie Institution for Science Department of Plant Biology. "Locus: AT5G44635." tair. National Science Foundation, 11 Feb. 2013. Web. 16 May 2013.
[8] "SPELL - S. Cerevisiae." SPELL - S. Cerevisiae. Stanford University, 2007. Web. 16 May 2013.
Page created by: Renae Geier, undergraduate student in genetics at the University of Wisconsin - Madison ([email protected])
Page last updated: 8/26/2013
Page last updated: 8/26/2013