PROTEIN NETWORKS
Below are some links to protein interaction networks as well as some images depicting the evidences towards these conclusions.
BioGRID 3.2 MCM6 Protein Interactions (D. melanogaster)
DroID-The Drosophila Interactions Database for MCM6
Reactome
BioGRID 3.2 MCM6 Protein Interactions (D. melanogaster)
DroID-The Drosophila Interactions Database for MCM6
Reactome
Figure 1: This image is of the STRING protein interaction network for MCM6.
Table 1: This table shows the MCM6-protein interactions in Drosophila melanogaster and the assay by which the interaction was determined [1]. I included this image to show that multiple methods have been used to determine these interactions, and the assays are variable.
From Figure 1 and using the information I gathered from STRING:
GMNN: Geminin, a DNA replication inhibitor that prevents the MCM complex from being loaded into the pre-replication complex. It is normally degraded during mitosis.
CDC45: required for the initiation of DNA replication
MCM10: brings MCM2-7 together with DNA polymerase
ORC2L: origin recognition complex, binds to the origin of replication
CDT1: chromatin licensing and DNA replication factor 1, required for loading MCM2-7 into the pre-replication complex (likely that geminin inhibits CDT1)
ANALYSIS AND DISCUSSION
It comes as no surprise to me that MCM6 is found to interact with other MCM proteins, since it is known that MCM2-7 for the eukaryotic replicative helicase [2]. It also comes as no surprise that MCM6 interacts with the other proteins mentioned by STRING. They are all required for DNA replication and replication initiation (or inhibition of this process) and MCM6 is involved in the entire process of replication.
The only modification of MCM6 that I am aware of is that it forms a hexamer with the other MCM2-7 proteins.
GMNN: Geminin, a DNA replication inhibitor that prevents the MCM complex from being loaded into the pre-replication complex. It is normally degraded during mitosis.
CDC45: required for the initiation of DNA replication
MCM10: brings MCM2-7 together with DNA polymerase
ORC2L: origin recognition complex, binds to the origin of replication
CDT1: chromatin licensing and DNA replication factor 1, required for loading MCM2-7 into the pre-replication complex (likely that geminin inhibits CDT1)
ANALYSIS AND DISCUSSION
It comes as no surprise to me that MCM6 is found to interact with other MCM proteins, since it is known that MCM2-7 for the eukaryotic replicative helicase [2]. It also comes as no surprise that MCM6 interacts with the other proteins mentioned by STRING. They are all required for DNA replication and replication initiation (or inhibition of this process) and MCM6 is involved in the entire process of replication.
The only modification of MCM6 that I am aware of is that it forms a hexamer with the other MCM2-7 proteins.
REFERENCES
[1] "Gene DmelMcm6." FlyBase Gene Report: DmelMcm6. FlyBase, 23 Jan. 2013. Web. 07 Mar. 2013. <http://flybase.org/reports/FBgn0025815.html>.
[2] Bochman, M. L., and A. Schwacha. "The Mcm Complex: Unwinding the Mechanism of a Replicative Helicase." Microbiology and Molecular Biology Reviews 73.4 (2009): 652-83. American Society for Microbiology. Web. 8 May 2013.
[1] "Gene DmelMcm6." FlyBase Gene Report: DmelMcm6. FlyBase, 23 Jan. 2013. Web. 07 Mar. 2013. <http://flybase.org/reports/FBgn0025815.html>.
[2] Bochman, M. L., and A. Schwacha. "The Mcm Complex: Unwinding the Mechanism of a Replicative Helicase." Microbiology and Molecular Biology Reviews 73.4 (2009): 652-83. American Society for Microbiology. Web. 8 May 2013.
Page created by: Renae Geier, undergraduate student in genetics at the University of Wisconsin - Madison ([email protected])
Page last updated: 5/16/2013
Page last updated: 5/16/2013